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Northwestern mass spectrometry

The Mass Spectrometry Facility supports Northwestern Research by performing both qualitative and quantitative analysis of small molecule, macro-molecules, and mixed mode biopolymeric samples. Examples of application areas are drug discovery, synthetic reaction monitoring, nano-particle characterization, etc Proteomics Northwestern Center Core. The Thermo Scientific™ TSQ Quantiva™ Triple Quadrupole Mass Spectrometer uses active ion management to exceed even the most stringent analytical requirements with superb sensitivity, speed, and dynamic range, enabling the need for ever-lower limits of d Top Down Mass Spectrometry is a technique to characterize the entire sequence of a protein with its related modifications. This service line is tailored to the comprehensive analysis of semi-pure proteins with minimal non-target protein contamination. Northwestern/CBC: Starting at $202 (depending on number of samples submitted and length of. Accurate Mass Analysis / Forumula Verification. Purity analysis of samples using LC-UV-MS. Compound molecular formula determination and/or confirmation. Quantitation of organic compounds in biological or environmental samples. Peptide / Protein molecular weight determination. Oligonucleotide molecular weight determination and impurity analysis

Dr. Ben Owen . Mass Spectrometry Director, Research Associate Professor Office: Tech BG53B Phone number: 847-491-2962 benjamin.owen@northwestern.ed IMSERC Mission. The Northwestern University (NU) Integrated Molecular Structure Education and Research Center (IMSERC) was established to educate NU students and researchers to be scientific leaders, support world-class research, and provide access to and education for students on the use of a variety of analytical instrumentation and methodologies Keck-II hosts Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS), X-ray Photoelectron Spectroscopy (XPS), Fourier Transform Infrared Spectroscopy (FT-IR), Confocal Raman System, High Resolution Stylus Profilometer, 3D Optical Microscope, Spectroscopic Ellipsometer, and Zetasizer

Clinical Pharm Home Page. Mission. The mission of the Mary Beth Donnelley Clinical Pharmacology Core Facility is to provide state-of-the-art quantitative mass spectrometry-based support to Northwestern University investigators as an integral part of bench-to-bedside drug development Proteomics Northwestern Center Core. Quantitation requires a consultation to help design the experiment. Depending on the problem, we can use a variety of methods to perform the analysis Bioinformatics Analysis of Top-Down Mass Spectrometry Data with ProSight Lite. In this tutorial, we show the analysis of intact protein spectra through deconvolution, deisotoping, and searching with ProSight Lite, a free, vendor-agnostic tool for the analysis of top-down mass spectrometry data We have been successful in driving both technology development and applications of high performance mass spectrometry at the interface of chemistry and biology. Over the last decade, we have generated ~120 publications, ~50 Ph.D. students/postdoctoral trainees, and ProSight software via the web to over 650 labs worldwide

Mass spectrometry - Northwestern Universit

Peptide Core Home Page Order from the Peptide Core. Mission. The mission of the Peptide Synthesis Core is to provide custom peptide synthesis, purification and mass spectrometry-based characterization services and technical support for members of the Simpson Querrey Institute (SQI), other Northwestern University researchers, and members of the Chicago and regional research community Neuropharmacology. Neuropharmacology is the study of how drugs affect the molecular, cellular or behavioral functions of the central and peripheral nervous system. Faculty in our department use modern tools of neuroscience to investigate fundamental brain processes implicated in several neurological disorders — including epilepsy, addiction.

Northwestern Proteomics - Mass Spectrometer

  1. This training in high performance mass spectrometry and enzymology explains much of the research performed by his independent laboratory over the last decade at the University of Illinois in Urbana-Champaign. In 2010 the Kelleher Group relocated to Northwestern University where the three main sub-groups continue working in the areas of Top Down.
  2. The National Resource for Translational and Developmental Proteomics (NRTDP) is dedicated to accelerating a significant shift in how protein molecules are analyzed by mass spectrometry, with a focus on intact protein measurements. This shift in approach propels a variety of national interests in technology development, systems biology, and.
  3. g their representative spectrum. Courtesy of Luis Schachner. Northwestern Medicine scientists have developed a new method to analyze the protein composition of intact nucleosomes without losing combinatorial information present in chromatin
  4. HIGHLIGHTED RESOURCE PUBLICATIONS. Kafader JO, Durbin KR, Melani RD, Des Soye BJ, Schachner LF, Senko MW, Compton PD, Kelleher NL. Individual Ion Mass Spectrometry Enhances the Sensitivity and Sequence Coverage of Top-Down Mass Spectrometry J Proteome Res. 2020. 19(3) p. 1346-1350 PubMed
  5. We have used mass spectrometry-based proteomics to detect bacterial and fungal secondary metabolites as well as their intermediates. Related Publications. Evans BS, Zhao C, Gao J, Evans CM, Ju KS, Doroghazi JR, van der Donk WA, Kelleher NL, Metcalf WW Discovery of the antibiotic phosacetamycin via a new mass spectrometry-based method for.
  6. This in-house modified system from Thermo Fisher is an integrated hybrid mass spectrometer consisting of a Velos Pro dual pressure quadrupole linear ion trap combined with a Fourier Transform Ion Cyclotron Mass Spectrometer. The instrument delivers ultra-high resolution (~1.7 million at m/z 400) accurate mass (< 1PPM), and high dynamic mass range
  7. Detection of hepatocellular carcinoma in a high-risk population by a mass spectrometry-based test Devalingam Mahalingam * , Leonidas Chelis, Imran Nizamuddin, Sunyoung S. Lee, Stylianos Kakolyris, Glenn Halff, Ken Washburn, Kristopher Attwood, Ibnshamsah Fahad, Julia Grigorieva, Senait Asmellash, Krista Meyer, Carlos Oliveira, Heinrich Roder.
Thermal analysis

Northwestern Proteomics - Top Down Mass Spectrometr

  1. A Researcher's Guide to Mass Spectrometry-Based Proteomics. Former postdoc JP Savaryn and current graduate student Tim Toby have published a tutorial on the ion physics underlying mass spectrometers to be intuitive for general readers in basic or applied biomedical research
  2. Top-down Proteomics is the analysis of intact proteins by mass spectrometry. This technique allows for exact mass calculations of protein analytes and is thus a reliable method for characterizing proteoforms. Specialized top-down techniques allow for analysis of folded proteins and their non-covalent binding partners, which provides a means for.
  3. We report a novel platform [native capillary zone electrophoresis-top-down mass spectrometry (nCZE-TDMS)] for the separation and characterization of whole nucleosomes, their histone subunits, and post-translational modifications (PTMs)
  4. The course is intended for career faculty, postdoctoral fellows, late-stage graduate students and industry partners with prior experience in mass spectrometry. Dates: TBD. Participants: 6-10 invited attendees. Cost: $1000 (academic) / $2000 (corporate) Location: Silverman Hall, Northwestern University, 2170 Campus Drive, Evanston, IL, 6020
  5. We present a method that combines immunoprecipitation of KRAS4B with top-down mass spectrometry (IP-TDMS), thus enabling the precise characterization of intact KRAS4B proteoforms. We provide detailed protocols for the IP, LC-MS/MS, and data analysis comprising a successful IP-TDMS assay in the contexts of cancer cell lines and tissue samples
  6. Northwestern University has a strong history of interdisciplinary and interdepartmental research in the life sciences and engineering, from which these fields have emerged as powerful opportunities for students. Mass spectrometry and proteomics Cluster Activities:.
  7. Precise Characterization of KRAS4B Proteoforms by Combining Immunoprecipitation with Top-Down Mass Spectrometry Lauren M. Adams, Caroline Jane DeHart, Neil L. Kelleher * * Corresponding author for this wor

Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) is being used to develop multi-element 2D and 3D images of singe cell and tissues. LA-ICP-MS provides many beneficial capabilities including low detection limits (µg g-1), full elemental mass spectrum coverage, high spatial resolution, limited sample preparation, wide. Northwestern University Feinberg School of Medicine. William Funk Lab Mass Spectrometry. Agilent 6490 QQQ mass spectrometer with a nano-Chip LC interface Chromatography. Two Agilent 1260 Infinity HPLC systems (one interfaced with the 6490 QQQ and one off-line), and a nano-Chip LC MS interface..

The Q Exactive LC/MS is used for precise measurements of targeted and non-targeted analytes using reverse-phase liquid chromatography mass spectrometry. This instrument is applied to determine the identity and abundance of intracellular and extracellular metabolites in order to monitor bacterial metabolism and contaminant degradation Imaging MALDI mass spectrometry, robotic liquid handling, high-throughput experiments, solid phase peptide synthesis, microfluidics, cloning, protein expression, purification, and characterization. Northwestern University is an Equal Opportunity, Affirmative Action Employer of all protected classes, including veterans and individuals with. The Mrksich Group uses the tools of organic chemistry, materials science and biochemistry to address important challenges in and at the intersection of chemistry and biology.. In one program, we emphasize the development of methods to perform high throughput experiments by combining engineered surfaces and mass spectrometry in a technique known as SAMDI-MS

Mass Spectrometry Specialist. Phone number: 847-497-1240 Office location: Tech BG90 arsen.gaisin@northwestern.edu. Michael Gerty. Co-manager of Northwestern University Laser and Electronics Design Core Facility (NU-LED) Phone number: 847-467-6968 Office location:. Attachment of process mass spectrometer gives quantitative analysis of, for example, the evolution of H2O during reduction of oxides, giving insight into possible structures of surface oxides. • Micromeritics ASAP 2010 surface area and porosity analyzer: This machine is currently plumbed with N2, CO2, and He and has micropore capability for.

(1) Expertise in mass spectrometry-based methods is required; experience with microbiology and molecular biology is a plus; (2) Expertise in molecular spectroscopic techniques for solid-state analysis OR molecular mmodeling simultaitons is required; experience with mineral surfaces is a plus)

Electron donor-acceptor systems • photochemistry • molecular electronics • surface chemistry of graphene and polymer nanocomposites • self-assembled monolayers • mass spectrometry. Dichtel, Kalow, Nguyen, Wasielewski. Total Synthesis & Method Developmen Faculty Director, Northwestern Proteomics, Chemistry of Life Processes Institute. Contributing to both technology development and application of mass spectrometry in chemistry and biology, The Kelleher Research Group is interested in the biosynthesis and discovery of novel natural products with potential pharmacological activities. The lab.

Introduction to principles and practice of organic and inorganic synthetic compound characterization by nuclear magnetic resonance spectroscopy (NMR) and mass spectrometry (MS). Topics include NMR instrument operation, spectra interpretation, 2-dimensional NMR spectroscopy, MS ionization and detection schemes, gas chromatography MS, liquid. Robert H. Lurie Comprehensive Cancer Center of Northwestern University ; Clinical Cancer Center Galter Pavilion 675 N. St. Clair, 21st Floor Chicago, IL 60611 866-LURIE-CC or 312-695-0990; Chicago Administrative Office 303 E. Superior L3 - 125 Chicago, Illinois 60611 312-908-5250 cancer@northwestern.ed The northwestern blot, also known as the northwestern assay, is a hybrid analytical technique of the western blot and the northern blot, and is used in molecular biology to detect interactions between RNA and proteins.A related technique, the western blot, is used to detect a protein of interest that involves transferring proteins that are separated by gel electrophoresis onto a nitrocellulose. Modern mass spectrometry for synthetic biology and structure-based discovery of natural products. Henke MT and Kelleher NL. Natural Product Reports. 2016 August 1;33(8):942-950. View all publications by Neil L. Kelleher listed in the National Library of Medicine (PubMed). Current and former IBiS students in blue.

(PDF) Eppawala-AP, Sri Lanka, an Apatite Standard

MS Services at IMSERC - Northwestern Universit

Project collaborators will look at the proteomics of pediatric brain tumors, liver transplant rejection, aging, heart failure, and Alzheimer's and Parkinson's disease, to name a few. These efforts, in turn, will drive technology development and applications for NRTDP's new style of mass spectrometry-based proteomics The National Resource for Translational and Developmental Proteomics (NRTDP) is accelerating the burgeoning revolution in how protein molecules are analyzed by mass spectrometry with the direct measurement of intact proteins by mass spectrometry The following protocols and standard operating procedures are used by our National Resource for top down proteomics research. Protocol SOP_001. For Generating TD LC-MS Data on Protein Standards. This standard protocol details how to make a standard mix of proteins for tracking LC-MS performance and illustrates how to configure an instrument for. A Northwestern Proteomics Collaborator is a physician/scientist who uses proteomics as a tool to increase their understanding of the world around us. They may not have a mass spectrometer within their research group, but they are well-versed in the language of proteomics Secondary ion mass spectrometry (SIMS) is a surface analysis tool that involves the removal of atoms from the sample surface. By measuring the mass and charge of the atoms removed from the surface, elemental, isotopic, and molecular information of the constituents present within a few nanometers from the surface can be identified

IMSERC staff - Northwestern Universit

Specific techniques explored include gas and high performance liquid chromatography, uv/visible spectroscopy, FTIR and Raman spectroscopy, elemental analysis by ICP atomic emission spectroscopy, and mass spectrometry A new technology developed at Northwestern University now offers some clarity to scientists with precision measurements of proteins down to their atoms. The powerful new approach, called individual ion mass spectrometry, or I2MS, can determine the exact mass of a huge range of intact proteins. It weighs each and every molecule on an individual. Particularly areas of interest include hydrogen exchange mass spectrometry of proteins and machine learning/deep learning. Staff Positions. 5/1/2021 - Research Technologist 1 External Applicants Apply Here: Job ID 39519 Internal Applicants Apply Here: Job ID 39519 The Mrksich group at Northwestern University is seeking a Research Technologist 1. A proteoform is a defined form of a protein derived from a given gene with a specific amino acid sequence and localized post-translational modifications. In top-down proteomic analyses, proteoforms are identified and quantified through mass spectrometric analysis of intact proteins. Recent technolog

Dr. Kelleher has been successful in driving both technology development and applications of very high performance mass spectrometry in both chemistry and biology. He has over 3 3 0 publications, an H-factor of 71, and provides ProSight software via the web to over 20 00 labs around the world. The core of the Kelleher Team is built around. Native mass spectrometry (nMS) is a technique growing at the interface of analytical chemistry, structural biology, and proteomics that enables the detection and partial characterization of non-covalent protein assemblies. Currently, the standardization and dissemination of nMS is hampered by technical challenges associated with instrument. Jacek worked in the proteomics core until 2010 where was involved in many collaboration projects which used mass spectrometry in qualitative as well as quantitative analyses. His Ph.D. thesis focused on mass spectrometry based, label free, quantitative analysis of yeast mitochondria proteins, using the LTQ-FT mass spectrometer custom QE-HF mass spectrometer (Thermo) or resolved by RPLC and subjected to denaturing TDMS on a custom LTQ-Velos21T FT-ICR mass spectrometer (NRTDP) at Northwestern University. Preliminary analysis of endogenous p53 populations by denaturing top-down mass spectrometry Creation of model p53 proteoforms: Visualization of rp53 phosphorylated.

Gabrielse, Northwestern University. Board of Trustees Professor Gerald Gabrielse, one of the world's leading practitioners of fundamental, low energy physics and an active member of the National Academy of Sciences, is relocating from Harvard to Northwestern to be the founding director of the Center for Fundamental Physics at Low Energy (CFP. Quantitation of carbohydrate monomers and dimers by liquid chromatography coupled with high-resolution mass spectrometry. Carbohydrate Research, 468, 30-35. # Co-first authors doi: 10.1016/j.carres.2018.08.007. (e-pub 2018 Aug 12) [Link to Article

IMSERC - Northwestern Universit

KECK-II - Home NUANCE: Northwestern University Atomic

Mary Beth Donnelley Clinical Pharmacology Core Facility

EVANSTON, Ill. --- Neil Kelleher, the Walter and Mary E. Glass Professor of Molecular Biosciences, professor of chemistry in the Weinberg College of Arts and Sciences and director of the Proteomics Center of Excellence at Northwestern University, has been awarded a $1 million grant from the W. M. Keck Foundation. The grant will fund Kelleher's development of a hybrid mass spectrometer, which. A good grasp of mass spectrometry fundamentals - particularly as related to orbitrap, triple quad, and time-of-flight mass spectrometers. Northwestern University 4.1. Chicago, IL 60642 (Noble Square area) 4 years' mass spectrometry experience in metabolomics, proteomics,. BMBS COST Action BM1104. Mass Spectrometry Imaging: New Tools for Healthcare Research. IMPORTANT: This COST action is finished. Mass spectrometry imaging is a rapidly developing technique that uses spatially resolved proteomic and metabolomic techniques to simultaneously trace the distributions of hundreds of biomolecules directly from patient tissue samples

Northwestern Proteomics - Hom

Home ProSight Lit

Jane is working on incorporating her backgrounds in protein kinetic stability and proteomic mass spectrometry into the Rocklin lab's high-throughput protein stability hydrogen-deuterium exchange (HDX) mass spectrometry assay. Her focus involves peptide and point mutation localizations within the protein libraries of the HDX project TITLE: A non-derivatized method for simultaneous quantitation of proteinogenic, urea-cycle, and acetylated amino acids by liquid chromatography-high-resolution mass spectrometry -ABSTRACT: The quantification of amino acids as freely dissolved compounds or from hydrolyzed peptides and proteins is a common endeavo

Neil Kelleher: Department of Chemistry - Northwestern

The Q Exactive LC/MS is used for precise measurements of targeted and non-targeted analytes using reverse-phase liquid chromatography mass spectrometry. This instrument is applied to determine the identity and abundance of intra and extracellular metabolites in order to monitor bacterial metabolism and contaminant degradation Chemistry of Life Processes Institute at Northwestern University is where new cures and biomedical discoveries begin. From landmark Alzheimer's studies to revolutionary new treatments for cancer, Parkinson's, cardiovascular disease and addiction, CLP Institute offers new hope to millions of people around the world living with disease New Method Allows Minimally Invasive Cell Sampling. Researchers developed one of the first non-destructive methods of extracting multiple samples from a cell. At any given moment, a variety of dynamic processes occur inside a cell, with many developing over time. Because current research methods for gene profiling or protein analysis destroy.

Northwestern Proteomics - Proteomics Center of Excellence

  1. The pair, who lead Northwestern's Center for Synthetic Biology, where Mrksich is the director and Jewett is the co-director, wondered what they could accomplish if they combined the Mrksich lab's mass spectrometry technology with the Jewett lab's expertise in glycosylation and rapidly making proteins
  2. We use biochemistry with discovery-based mass spectrometry (MS) to identify the protein perturbations which drive synaptopathies and proteinopathies. Groups of perturbed proteins serve as pathway beacons which we subsequently characterizes in hopes of finding new pathogenic mechanisms and potential future therapeutic targets
  3. CHEM 350-1 Advanced Laboratory 1 (1 Unit) Advanced laboratory techniques in synthetic and analytical chemistry and spectroscopy: mass spectrometry, chromatography, NMR spectroscopy, and organic synthesis techniques. Prerequisites: CHEM 220-0 and CHEM 212-3 (C- or better) or equivalent
  4. For example, we have developed the SAMDI method, which uses mass spectrometry to analyze the arrays, and we have extended this method to the first label-free approach for high throughput screening, to the functional annotation of recently sequenced genes and towards an understanding of the networks that regulate protein acetylation
Category:Tools - Northwestern University Center for Atom

Peptide Synthesis Core: Research: Feinberg School of

  1. J Am Soc Mass Spectrom. 2017 , 28, 1203-1215. Elucidating the rimosamide-detoxin natural product families and their biosynthesis using metabolite/gene cluster correlations
  2. Kelleher's team developed a four-dimensional separation system that uses separations and mass spectrometry to measure the charge, mass and weight of each protein as well as how greasy a protein is
  3. We tackle these questions with a myriad of methods: forward genetics; fluorescence microscopy; next-generation sequencing; virology; protein mass-spectrometry; cell culture; computer modeling. We work with collaborators in the Chicago area and around the world. Non-Coding RNAs. Gene Regulation. Tissue-Scale Self-Organization of Cells
  4. A mass spectrometer ultimately measures the mass (m) of a molecule in relation to its charge (z). In the macroscopic world we measure the mass of something using a scale and the force of gravity. For example, a hypothetical dog is composed of 18 kg of mass. or Northwestern University. Footnotes. Conflict of Interest
  5. Franz M. Geiger is a Professor of Chemistry at Northwestern University. He utilizes cutting-edge laser spectroscopy, mass spectrometry, and computational techniques to focus on the special role that surfaces and interfaces play in geochemistry, atmospheric chemistry, and biophysics.More informatio

Neuropharmacology - Northwestern Universit

Northwestern Home | Northwestern Calendar: Plan-It Purple | Northwestern Search Kelleher Research Group 2145 N. Sheridan Road Evanston, IL 60208 Phone: 847.467.1076 Fax: 847.467.3276 General Inquirie While at Northwestern, he worked on advanced mass spectrometry-based-methods to study peptides and proteins. In the summer of 2002, he moved to Indiana University as Professor and a Robert & Marjorie Mann Chair in the Department of Chemistry Temporal Sampling of Enzymes from Live Cells by Localized Electroporation and Quantification of Activity by SAMDI Mass Spectrometry Prithvijit Mukherjee , Department of Mechanical Engineering, Northwestern University, Evanston, IL, 60208 US When asked whether they would transition all clinical mass spectrometry testing to a closed, fully automated Food and Drug Administration (FDA)-approved LC-MS/MS system if one became available, only one respondent would choose replacement. FAACC, a researcher and associate professor of pathology at Northwestern University Feinberg School of.

Neil Kelleher - Northwestern Universit

Schoof of Engineering and Applied Science, Northwestern University, Evanston. Hinois 60208-3108 (Received 7 July 1993; accepted for publication 8 August 1993) The mass resolution of the atom-probe field-ion microscope is limited by the time resolution of the ion-separating spectrometer Reach for the Stars: Computational Models for Teaching and Learning in Physics, Astronomy and Computer Science is a GK-12 program funded by the National Science Foundation, with support from CIERA and Northwestern University.. This NSF program places STEM graduate student fellows in K-12 science classrooms for the academic year with the goal of enriching their education and strengthening their. I received an MD from the Northwestern University Feinberg School of Medicine, where I also completed residency and chief residency in internal medicine. I then undertook fellowship training in pulmonary and critical care medicine at the Johns Hopkins University School of Medicine before returning to Northwestern as a faculty member in 2015 Mission. The Robert H. Lurie Comprehensive Cancer Center (RHLCCC) Flow Cytometry Core Facility (Lurie Flow Core) was established in 1992 to serve the needs of the Cancer Center, Northwestern University Feinberg School of Medicine (FSM), Northwestern University and other affiliated institutions whose studies require the measurement of selected characteristics of intact cells in a mixed.

Northwestern University - Our Missio

In his last year of undergraduate school at Northwestern University, Stephen Shrader, Ph.D. had the great fortune of taking a computer programming class, the first ever offered at the University. Later at Wayne State University as a graduate student in the field of mass spectrometry, a brand new course of study at that hallowed institution. ‪Research Associate, Northwestern University‬ - ‪‪Cited by 268‬‬ - ‪Mass spectrometry‬ - ‪proteomics‬ - ‪histones Michael Rourke, a doctoral chemistry researcher at Northwestern University, is advancing organic chemistry research with the OT-2 and Protocol Designer. We coupled this with liquid chromatography mass spectrometry (LCMS), as well as gas chromatography mass spectrometry (GCMS), so I can put a standard in it before I run the reaction, or I.

Proteomics in Pathology: Call for Papers for Upcoming

South Side Chicago Northwestern Neuroscience Internship Program: We then researched each protein that was gathered in the mass spectrometry analysis to find if they had any association to Alzheimer's Disease and if there was a significant difference in protein expression between the control patient and the two Alzheimer's patients. From. Name: Swanson, Geoffrey. Title: Professor. Email: gtswanson@northwestern.edu. Office Phone: 312-503-1052. Department: Pharmacology; Director, NUIN. Office: Lurie 8-11 Proteoform Atlas for Imaging Mass Spectrometry RTI Northwestern & TMC Vanderbilt (Kidney) Refining informatic approaches to enhance top-down proteomics data in the HuBMAP Consortium RTI Northwestern & TTD PNNL. Spatial and Cell-type Restriction of Human CD45 Proteoforms RTI Stanford & RTI Northwestern Ion Mobility Spectrometry, online course of 8 lectures taught at the Moscow Engineering Physics Institute Spring 2016 Analytical Chemistry (CHEM 523, 4 credits) Textbook D.C. Harris, Quantitative Chemical Analysis Fall 2014 (50 students), Fall 2015 (39 students), Fall 2017 (35 Students) Mass Spectrometry (CHEM 809B, 3 credits